LOCUS       sequence-90013-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 GSSGSS NusA TEV LIC cloning vector (2...
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             176..198
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             218..235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gtgccggctc
      121 cggagagctc tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt
      181 ttaactttaa gaaggagata tacatatgaa atcttctcac catcaccatc accatggttc
      241 ttctggttct tctggttctt ctatgaacaa agaaattttg gctgtagttg aagccgtatc
      301 caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac
      361 agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag
      421 cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa
      481 ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt
      541 tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt
      601 tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca
      661 cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga
      721 tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt
      781 ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc
      841 gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt
      901 gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg
      961 tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg
     1021 tatgcgtggc gcgcgtgttc aggcggtgtc tactg
//
