LOCUS       sequence-90019-e        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 High pI protein 1 TEV LIC cloning vec...
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..220
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     304..323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        304..322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             411..433
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             453..470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1074..1094
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
       61 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      121 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      181 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      241 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      301 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      361 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac
      421 tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat ggttcttcta
      481 tggagaagcc aaaagtagaa aggtctgaag gtaaggaaaa acttgaggat tacgcttatg
      541 tacttgattt catgccatac ggtcatcctg acgataaaag gccaatacac aggagggagc
      601 cgctcgcaca agtcgttggg gagagaaact ttacacttct cgaggttagc ataagaaagg
      661 gtaagcagcc cctggtaatg gacagagttt acatcggcaa gggggagaga gatgtagtgt
      721 acaagatcaa gagaaggctg agatacgagg atttaactcc tgccgcaaag actgagctgc
      781 cttacgttat cgagcacata ataaagcagg atgagaagaa gtacgtggat ttcttcaaca
      841 aggccgattc cataacaacg agaatgcacc agctcgagct tcttcccggc gttggaaaga
      901 agatgatgtg ggcaataatc gaggagagga aaaagaggcc ctttgagagt tttgaagaca
      961 tagcccagag agtcaagggc attcagaggc ccgaaaagct aatcgtcagc agaattatat
     1021 acgaaatcaa gaatcctcaa accaagtaca agctctttac tgctgggatc gaggaaaacc
     1081 tgtacttcca atccaatatt ggaagtggat aacggatccg cga
//
