LOCUS       sequence-90027-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 High pI protein 1 TEV LIC cloning vec...
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(62..67,68..84,85..90)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(456..503)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     499..517
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(642..662)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctcatg
       61 tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac
      121 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      181 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
      241 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
      301 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
      361 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
      421 tcctgtggat atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
      481 gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
      541 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
      601 ggcgcgccgc gatcgcggat ccgttatcca cttccaatat tggattggaa gtacaggttt
      661 tcctcgatcc cagcagtaaa gagcttgtac ttggtttgag gattcttgat ttcgtatata
      721 attctgctga cgattagctt ttcgggcctc tgaatgccct tgactctctg ggctatgtct
      781 tcaaaactct caaagggcct ctttttcctc tcctcgatta ttgcccacat catcttcttt
      841 ccaacgccgg gaagaagctc gagctggtgc attctcgttg ttatggaatc ggccttgttg
      901 aagaaatcca cgtacttctt ctcatcctgc tttattatgt gctcgataac gtaaggcagc
      961 tcagtctttg cggcaggagt taaatcctcg tatctcagcc ttctcttgat cttgtacact
     1021 acatctctct cccccttgcc gatgtaaact ctgtccatta ccaggggctg cttacccttt
     1081 cttatgctaa cctcgagaag
//
