LOCUS       sequence-90034-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        416..520
                     /gene="bla"
                     /label=AmpR promoter
     promoter        522..879
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        522..713
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(545..565)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      730..865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctgatt taaataggag atcatgcatc
       61 cagatctggt gataggtggc aagtggtatt ccgtaaggat atcgttttcg ggagtagtgc
      121 cccaactggg gtaacctttg agttctctca gttgggggcg tagggtcgcc gacgttttcg
      181 ggagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg tagggtcgcc
      241 gacgttttcg ggagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
      301 tagggtcgcc gacggatcct ttgtcgacga agttcctatt ccgaagttcc tattcttcaa
      361 aaggtatagg aacttcgcta gcgattattc aggtggcact tttcggggaa atgtgcgcgg
      421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      481 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
      541 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      601 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      661 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      721 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      781 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      841 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
//
