LOCUS       sequence-90036-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        411..515
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        517..708
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(540..560)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tgatttaaat aggagatcat gcatccagat
       61 ctggtgatag gtggcaagtg gtattccgta aggatatcgt tttcgggagt agtgccccaa
      121 ctggggtaac ctttgagttc tctcagttgg gggcgtaggg tcgccgacgt tttcgggagt
      181 agtgccccaa ctggggtaac ctttgagttc tctcagttgg gggcgtaggg tcgccgacgt
      241 tttcgggagt agtgccccaa ctggggtaac ctttgagttc tctcagttgg gggcgtaggg
      301 tcgccgacgg atcctttgtc gacgaagttc ctattccgaa gttcctattc ttcaaaaggt
      361 ataggaactt cgctagcgat tattcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      481 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
      541 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      601 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      841 aggaggcttt ttt
//
