LOCUS       sequence-90037-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    180..279
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        449..553
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        555..746
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(578..598)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ccctcactaa agggaacaaa agctggagct gatttaaata ggagatcatg catccagatc
       61 tggtgatagg tggcaagtgg tattccgtaa ggatatcgga tcttttctcg actagtactg
      121 acggacacac cgaagccccg gcggcaaccc tcagcggatg ccccggggct tcacgttttc
      181 ccaggtcaga agcggttttc gggagtagtg ccccaactgg ggtaaccttt gagttctctc
      241 agttgggggc gtagggtcgc cgacatgaca caaggggttg tgaccggggt ggacacgtac
      301 gcgggtgctt acgaccgtca gtcgcgcgag cgcgactagt cgagaaggat cctttgtcga
      361 cgaagttcct attccgaagt tcctattctt caaaaggtat aggaacttcg ctagcgatta
      421 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      481 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      541 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
      601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
      661 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      721 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
      781 cccagttccg cccattctcc gccccatggc tgactaattt tttt
//
