LOCUS       sequence-90039-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for PhiC31 integrase-mediated cassette exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT378_pattB-pCA-GFP-pA-attB
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    228..297
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    360..481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(371..390)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     425..444
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(648..669)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccctcactaa agggaacaaa agctggagct ccactcgaca tgcccgccgt gaccgtcgag
       61 aacccgctga cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc
      121 accgtgacca ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc
      181 gccgggatca actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg
      241 gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc
      301 gtgacctaca tcgtcgagtg gcggccgctc tagaactagt tgggcgcgcc caatttaaat
      361 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      421 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      481 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      541 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagaat
      601 tcttacttgt aaagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      661 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
      721 tagtggttgt cgggcagcag cacggggcc
//
