LOCUS       sequence-90040-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for PhiC31 integrase-mediated cassette exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT374_pattB-pCA-GFP-pA-attBSwa
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    275..344
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    368..489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(379..398)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     433..452
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(656..677)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa
       61 ctagttgggc gcgcccaatt ttcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc
      121 tgccccgcgt atccgcaccc gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg
      181 cgcccagcgg tttcgagggc gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact
      241 accgccacct cgacccgttc atcatgatgg accagatggg tgaggtggag tacgcgcccg
      301 gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagaccgtg acctacatcg
      361 tcgaaaataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      421 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      481 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      541 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta
      601 tctagaattc ttacttgtaa agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      661 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      721 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctg
//
