LOCUS       sequence-90042-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Phic31 integrase-mediated transgene integration;the bacterial 
            backbone can be subsequently removed by crossing to Flp-expressing 
            mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT344_pattB-pCA-GFP-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    78..199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(89..108)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     143..162
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(366..387)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggagctcc accgcggtgg cggcccgaag ttcctatacc ttttgaagaa taggaacttc
       61 ggcgcgccca atttaaataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      121 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      181 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      241 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      301 tgatcagtta tctagaattc ttacttgtaa agctcgtcca tgccgagagt gatcccggcg
      361 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      421 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      481 gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
      541 ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      601 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      661 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      721 ta
//
