LOCUS       sequence-90051-e        1139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TEV SNAPf Prescission TwinStrepII clo...
FEATURES             Location/Qualifiers
     source          1..1139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(190..195,196..212,213..218))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     602..619
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
       61 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      181 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
      241 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
      301 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
      361 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
      421 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
      481 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
      541 tggctacgta tactccggac tattaataga tcatggagat aattagcggc cgcttaatta
      601 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
      661 taaaaaaacc tataactatt ccggattatt cataccgtcc caccatcggg cgcggatccc
      721 tttaagaagg agatatagtt aacctctact tccaatccgg ctctgataaa gactgcgaga
      781 tgaagcgcac gacgctcgat tcgccccttg gcaaattgga actgagcggt tgtgagcaag
      841 gtctgcatcg tatcatcttt ctgggaaagg gaactagtgc agcagatgca gtcgaggtcc
      901 cggctcctgc tgccgtgctt ggtggtcccg agcctttgat gcaagctacc gcttggctga
      961 atgcatattt ccatcagcca gaggcgatcg aggagttccc cgttcccgcg ctccatcatc
     1021 cggttttcca acaggaatcc ttcacccgcc aggtcctgtg gaagctcctg aaggtcgtaa
     1081 agtttggtga agtgatctca tacagtcact tggccgccct ggcgggcaac ccagccgct
//
