LOCUS       sequence-90052-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TEV SNAPf Prescission TwinStrepII clo...
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(274..297)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(334..357)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(367..390)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1091..1108)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gtaaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagtactt ctcgacaagc ttggtaccgc atgcctcgag actgcaggct
      181 ctagattcga aagcggcccc taggagatca tgaggccgcg actagtgagc tcgtcgacgt
      241 aggcctttga attccgcgcg cttcggaccg ttatttctcg aattgtgggt gtgaccagct
      301 gccacctccg ctaccgccgc cgctgccacc gcctttctca aattgggggt gagaccatga
      361 agctcccggg ccctggaaca gtacctccaa accaccggcg ggtccaaggc caggtttgcc
      421 cagacgatgg ccctcatgtg ccaacagcca ttctttcaca gccagaccgc cctcgtaacc
      481 tcccacgtcc aggtcgcctt ggacgactct atgacaaggg atcaggatag gtacagggtt
      541 gccactgagt gcggtcttaa cggcagcagt agcggctggg ttgcccgcca gggcggccaa
      601 gtgactgtat gagatcactt caccaaactt tacgaccttc aggagcttcc acaggacctg
      661 gcgggtgaag gattcctgtt ggaaaaccgg atgatggagc gcgggaacgg ggaactcctc
      721 gatcgcctct ggctgatgga aatatgcatt cagccaagcg gtagcttgca tcaaaggctc
      781 gggaccacca agcacggcag caggagccgg gacctcgact gcatctgctg cactagttcc
      841 ctttcccaga aagatgatac gatgcagacc ttgctcacaa ccgctcagtt ccaatttgcc
      901 aaggggcgaa tcgagcgtcg tgcgcttcat ctcgcagtct ttatcagagc cggattggaa
      961 gtagaggtta actatatctc cttcttaaag ggatccgcgc ccgatggtgg gacggtatga
     1021 ataatccgga atagttatag gtttttttat tacaaaactg ttacgaaaac agtaaaatac
     1081 ttatttattt gcgagatggt tatcattttt aattaagcgg ccgctaatta tctccatgat
     1141 ctattaata
//
