LOCUS       sequence-90054-e        8339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human XPO5 on the C-terminus of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Seow Y, Sibley CR, Wood MJ
  TITLE     Artificial mirtron-mediated gene knockdown: functional DMPK 
            silencing in mammalian cells.
  JOURNAL   RNA. 2012 Jul;18(7):1328-37. doi: 10.1261/rna.030601.111. Epub 2012 
            May 30.
  PUBMED    22647847
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.030601.111"; journalName: "RNA"; date: "2012-07"; 
            volume: "18"; issue: "7"; pages: "1328-37"
COMMENT     Sequence Label: peGFP-XPO5
FEATURES             Location/Qualifiers
     source          1..8339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3856..3867)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4951..5003
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    5127..5248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5164..5183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5218..5237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5255..5710)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5392..5413)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5604..5623
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5737..5841
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5843..6200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5843..6034
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5866..5886)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6051..6186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6235..7029
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6235..7029
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6289..6308)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6899..6918
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7217..7236)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7261..7308
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7637..8225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8126..8145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggaatggc gatggatcaa gtaaacgcgc tgtgcgagca gctggtgaaa
     1381 gcggtgacgg tcatgatgga ccccaactcc acccagcgct accggctgga agccctcaag
     1441 ttttgtgagg agtttaaaga aaagtgtcct atctgtgtcc cctgtggctt gaggttggct
     1501 gagaaaacac aagttgccat cgtcagacat tttggccttc agatcctgga acacgttgtc
     1561 aagtttcggt ggaacggcat gtctcgattg gagaaggtgt atctgaagaa cagtgtcatg
     1621 gagctgattg caaatggaac attgaacatt ttggaagagg agaaccatat taaagatgct
     1681 ctgtctcgaa ttgtagtgga aatgatcaag cgagagtggc cacagcattg gcctgacatg
     1741 ctaatagaat tggacactct ttccaaacaa ggggaaacac agacagaatt ggtgatgttt
     1801 atccttttgc gactggcaga ggatgtagtg acttttcaga cacttccccc tcaaagaaga
     1861 agggacatcc agcaaacatt aacccagaac atggaaagga tcttcagttt tctgcttaac
     1921 acacttcaag aaaatgtaaa caagtatcag caagtgaaga cagatacttc tcaggagtca
     1981 aaggcgcaag caaactgtcg agtaggagtt gcagcactga atactctagc aggctatatt
     2041 gactgggtgt ctatgagtca catcactgct gaaaactgta aactcctgga gatactgtgt
     2101 ttgctgttga atgaacagga acttcagttg ggagccgctg agtgtcttct cattgcagtc
     2161 agcagaaaag gcaagttgga agaccggaag cccttgatgg tcttatttgg agatgttgcc
     2221 atgcattata tactctccgc cgcacagact gctgatggag gaggtttggt agaaaaacac
     2281 tacgtctttc tgaagaggct ctgtcaggtg ttgtgtgcgc tgggcaatca gctgtgtgca
     2341 ttgctgggtg cagattctga tgtagaaaca ccatcaaact ttggaaaata cctggaatct
     2401 tttcttgctt tcacaaccca tccaagtcag tttctacgct cttcaactca gatgacttgg
     2461 ggagccctct tcaggcatga aatcctgtcc cgtgatcctt tgctattagc aataatacca
     2521 aaatatcttc gtgcttccat gactaacttg gtcaagatgg gctttccttc taaaacagac
     2581 agccctagct gtgaatattc tcggtttgat tttgatagcg atgaggactt caatgctttc
     2641 ttcaactcct cccgagcaca acaaggagag gtgatgaggt tggcatgtcg tttggatccc
     2701 aaaactagct tccagatggc tggggagtgg ctaaagtatc aactatcaac ttttcttgat
     2761 gctggttctg tgaattcttg ttctgcagtt ggaactggag aaggaagcct ctgttccgtc
     2821 ttctcacctt cattcgtgca gtgggaagcc atgactcttt ttttggaaag tgttatcacc
     2881 cagatgtttc gaacactaaa tagagaagaa attcctgtta atgatggaat agagctattg
     2941 cagatggttc tgaactttga taccaaggat cccctcatcc tgtcctgcgt ccttactaat
     3001 gtctctgcac tctttccatt tgtcacctac agaccagagt tcctgcccca ggtcttctct
     3061 aagctatttt catctgtcac ttttgaaact gttgaagaaa gtaaggcccc cagaacccgg
     3121 gcagtgagga atgtgaggag gcatgcttgt tcctccatca tcaagatgtg tcgtgactac
     3181 ccccagcttg tgctgcccaa ttttgacatg ctttataacc atgtgaagca actcctctcc
     3241 aatgagctac tcctgacaca aatggagaag tgtgccctca tggaagccct ggttctcatt
     3301 agcaaccaat ttaagaacta cgagcgtcag aaggtgttcc tagaggagct gatggcacca
     3361 gtggccagca tctggctttc tcaagacatg cacagagtgc tgtcagatgt tgatgctttc
     3421 attgcgtatg tgggtacaga tcagaagagc tgtgacccag gcctggagga tccgtgtggc
     3481 ttaaaccgtg cacgaatgag cttttgtgta tacagcattc tgggtgtggt gaaacgaact
     3541 tgctggccca ctgacctaga agaggccaaa gctgggggat ttgtggtggg ttatacatcc
     3601 agtggaaatc caatcttccg taacccctgc acagagcaga ttctgaaact tcttgacaat
     3661 ttgcttgcgc ttataagaac ccacaataca ttatatgcac cagaaatgct agccaaaatg
     3721 gcagagcctt tcaccaaggc tctggatatg cttgacgcgg aaaaatctgc tatattagga
     3781 ttacctcaac ctctcttgga actcaatgac tctcctgtct tcaaaaccgt cttggaaaga
     3841 atgcagcgtt tcttctctac cctctatgaa aactgttttc atatcctagg gaaggcaggc
     3901 ccttccatgc agcaagactt ctatactgtg gaggaccttg ctacccagct tctcagctca
     3961 gcctttgtca acttgaacaa tattcctgac taccgactca gacccatgct tcgtgtcttt
     4021 gtaaagcctc tggtgctctt ctgtccccca gagcactatg aagccctggt atcccccatc
     4081 ctcggacctc ttttcaccta cctccatatg aggctttctc agaaatggca agttatcaac
     4141 caaaggagcc tgctgtgtgg agaagatgag gctgcagatg aaaacccaga gtctcaagag
     4201 atgctggagg agcaactggt gaggatgtta acccgagaag tcatggacct aatcacggtt
     4261 tgctgtgttt caaagaaggg tgctgaccac agtagtgctc ccccagcaga tggagacgat
     4321 gaagaaatga tggccacaga ggtcaccccc tcagctatgg cagagcttac agacctgggc
     4381 aaatgtctga tgaagcatga ggatgtttgt acagcgctat taattacagc cttcaattcc
     4441 ctggcctgga aagatactct gtcctgccag aggacaacct cacagctctg ctggcctctc
     4501 ctcaaacaag tgctgtcagg gacactgctc gcagatgcag ttacgtggct tttcaccagt
     4561 gtgctgaaag gcttacagat gcacgggcag cacgacgggt gcatggcttc cctggtccat
     4621 ctggccttcc agatatacga ggcactgcgc cccaggtacc tggagataag agctgtaatg
     4681 gagcaaatcc ctgaaataca gaaggactca ctggaccagt ttgactgcaa gcttttaaac
     4741 ccctccctgc agaaagtggc tgacaagcgc cgaaaggacc aattcaaacg cctcattgct
     4801 ggttgcattg ggaaaccctt gggagagcag ttccgaaaag aagttcacat taagaatctt
     4861 ccctcacttt tcaaaaaaac aaagccaatg ctggagacgg aggtgctgga caatgatggg
     4921 ggtggcctgg ccaccatctt tgaaccctga ctcgagctca agcttcgaat tctgcagtcg
     4981 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
     5041 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     5101 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5221 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     5281 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     5341 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     5401 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     5461 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     5521 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     5581 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     5641 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     5701 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5761 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5821 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     5881 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5941 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6001 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6121 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6181 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6241 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6301 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6361 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     6421 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6481 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6541 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6601 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6661 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6721 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     6781 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6841 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6901 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6961 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     7021 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     7081 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7141 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7201 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     7261 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     7321 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     7381 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     7441 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     7501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8281 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
