LOCUS       sequence-90060-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(269..288)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    313..360
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      574..583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
       61 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
      121 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
      181 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
      241 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
      301 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
      361 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
      421 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
      481 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcag
      541 gatcatctct agcctgcagg aattaagtta accgccacca tggataccta cgccggagcc
      601 tacgacagac agagccggga gagagagaac agcagcgccg ccagccccgc cacccagaga
      661 agcgccaacg aggataaggc cgccgatctg cagagagagg tggagaggga cggcggcaga
      721 ttcagatttg tgggccactt cagcgaggcc cctggcacca gcgccttcgg caccgccgag
      781 agacccgagt tcgagagaat cctgaacgag tgtagggccg gcaggctgaa catgatcatc
      841 gtgtacgacg tgtcccggtt cagcaggctg
//
