LOCUS       sequence-90064-e        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JMJD1BA-c068
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttccc tgcgaattcc tgagtcaggc
      181 ggaaacagtg ctttacatgt tctggagata caaagtcttc tgctactttt atgcaactgt
      241 atagattgtg aacctggtgt ggggctccag caggtatgaa aacagcatca cctaggaact
      301 gcacaatagc ccagccttgc acgccatact cctcatagag tcgcttacgg agggtctggt
      361 ccaggtacca actttggtca tgaattgggt catgatcagg ggggttctct tggccttgtt
      421 cttctccaac ctttcggagc agctcccgga tcttctctgc atccttggct gcatagatgt
      481 gccataaagc acctggcttc tcttttccat catgaatcct ctgcttcgtc acctcatcgg
      541 catctccctc gtcaattgtc ttgagtacct cttcatcatg agcaccctcc ccgatgggaa
      601 tcccaacata caccatcaca ttaacagcat cagacacatc taagtgaaga tttgttgtac
      661 caactcttct atcttctgct gttatcaacc cataggcgtt gtacatcttg gggcccagat
      721 caggccttac aaagtagcta ggtagcctag aggccagatt gagcctgcca tctcgtttgg
      781 tatattctgg cagaggaagg ttctccatca gatcttcaaa cctggttggc atcatgtctc
      841 gaaaatcttc cccaggaggc cagtccttga gtttgagcac cattggctgc ccatcttctg
      901 accgtagtcg tttgcatatg atctcgaaac catcccagaa atcccgaact ttcacatcgg
      961 aaattatagc acagttcctg cagttcacca agtctacatc ctggtctcca aattcctggc
     1021 taaaggcttc tggctt
//
