LOCUS       sequence-90065-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BLMA-c800
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(147..191)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(198..218)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcttcactt atcgtcgtca tccttgtaat
      121 cagatccgta tccaccttta ctgtcattcg tgccattcga ttttctgagc ctcgaagatg
      181 tcgttcagac cgccaccgga ttggaaatac aaattctcgg acatccctgg ggaactgtct
      241 tcagctggcg atgtccattc agagtatttc tgtaatactg aaatcacttc cgcaccatat
      301 ttttccagtt tgtcttcagt aacaccatca atttgaagca aaacctcagg atcagaagat
      361 aaagattctg caagcttctt gagagtgacg gtattaaaaa tattgaagta atggacacca
      421 aaaactttcc ccagagattt gcagacttct gtaagttctc caagacattt tttaaccatc
      481 tcttccctct gagacacttt tgctactaac gctttttgtt ttttcacact gctggaattt
      541 tctgtttcca taaagtctac ctttaaattg ccatttagta cagtttgggc tttatttccg
      601 agcatcacat aagcgatcgc ctggtcattg gcattgatat ataagtcttc atccaaaatc
      661 ttgtcaagta tcagcttttt aaaaagtctt tcggcattgt gtcgtgaata agcagatcct
      721 tttccaaata tacctgactg gatttttgca ctcttactcc ccaagaaaat gtcgaccagc
      781 atattcatag taaatcttcc agaaggacct acatgtttta tatttctcat tccttgtgat
      841 gaactatgtt cttgaacaaa tcttacaata cttttcacat cgtcagtcac atctcttgtt
      901 ttataatcc
//
