LOCUS       sequence-90069-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: INCENPA-c002
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(274..294)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(304..957)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(324..346)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(969..991)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    980..996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1004..1010,1011..1027,1028..1033))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        complement(join(1004..1010,1011..1027,1028..1033))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 acgcgcgagg cagatcgtca gtcagtcacg atgcggccgc tcgagtatcc acctttactg
       61 tcactgcagg ggcggtgagt tccagacagc agagctggtg cgcttgtgat agcggggctt
      121 gctcttcttg aagatatcct ccaagtccag tgggagaatg gttccaaaga gctccagaag
      181 gttcggtggg tggtagtact ggtgaatgat agcctggctg agcggggtgc ctcgggccca
      241 ggtggggatg ggcttccggg gatgggcctc catggattgg aagtacaggt tctctgagct
      301 cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca aaggccatgc
      361 tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa tacgtttttt
      421 aaaacaaact aattttggga acgcatccag gcacattggg tccatgtata aaacaacatc
      481 aagagcgtca tacaacatga agtcaggatg ggttacatga tcaccattta aatatgtttt
      541 atgacataaa cgatcttcga acattttcag catttcaggt agcttgctaa gaaaatcaac
      601 tttgagagtt tcaaagtctt tactatatgc aattctcgaa acaccgtatc taatatccaa
      661 aaccgctcct tcaagcattg aaatctctgc acgctctttt ggacaaccac ccaacatgtt
      721 gtgcttgtca gctatataac gtatgatggc catagactgt gttaatttaa catcaccatc
      781 aatataataa ggaagattgg gaaactccaa acccaattca aactttttgt ttcgccattt
      841 atcaccttca tcgcgctcat acaaatgctc ttcatatttt tcttcaagat attccaaaag
      901 aagtcgagtg ggttgcacaa ggcccttaat tttccaataa cctagtatag gggacatgaa
      961 tactgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagcccgat
     1021 gattaattgt caacagctca tttca
//
