LOCUS       sequence-90073-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac cloning vector with BioBrick PolyProm...
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(190..195,196..212,213..218))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     627..644
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     primer_bind     complement(1069..1088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
       61 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      181 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
      241 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
      301 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
      361 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
      421 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
      481 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
      541 tggctacgta tactccggac tattaataga tcatggagat aattagcggc cgcgtttaaa
      601 catccaccat ttgatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
      661 tactgttttc gtaacagttt tgtaataaaa aaacctataa ctattccgga ttattcatac
      721 cgtcccacca tcgggcgcgg atcctttatt aagtacttcc aatccaatat tggaagtgga
      781 taaccggtcc gaagcgcgcg gaattcaaag gcctacgtcg acgagctcac tagtcgcggc
      841 ctcatgatct cctaggggcc gctttcgaat ctagagcctg cagtctcgag gcatgcggta
      901 ccaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
      961 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     1021 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1081 caaatttcac aaataaagca ttttt
//
