LOCUS       sequence-90074-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac cloning vector with BioBrick PolyProm...
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  257..422
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    496..630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(520..539)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     574..593
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1018..1035)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
       61 ctacagggcg cgtcccattc gccattcagg ctgcaaataa gcgttgatat tcagtcaatt
      121 acaaacatta ataacgaaga gatgacagaa aaattttcat tctgtgacag agaaaaagta
      181 gccgaagatg acggtttgtc acatggagtt ggcaggatgt ttgattaaaa acataacagg
      241 aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg gtggaaatgg
      301 agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg tgtagcgtcg
      361 taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg
      421 ttcggatctc ccctgcaggc gtttaaacct aactccattt aaatggtgga tgtttctagg
      481 ctcaagcagt gatcagatcc agacatgata agatacattg atgagtttgg acaaaccaca
      541 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      601 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
      661 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
      721 atggctgatt atgatcctct agtacttctc gacaagcttg gtaccgcatg cctcgagact
      781 gcaggctcta gattcgaaag cggcccctag gagatcatga ggccgcgact agtgagctcg
      841 tcgacgtagg cctttgaatt ccgcgcgctt cggaccggtt atccacttcc aatattggat
      901 tggaagtact taataaagga tccgcgcccg atggtgggac ggtatgaata atccggaata
      961 gttataggtt tttttattac aaaactgtta cgaaaacagt aaaatactta tttatttgcg
     1021 agatggttat cattttaatt atctccatca aatggtggat gtttaaacgc ggccgctaat
     1081 tatctccatg atctattaat agtccggagt atacgtagcc aaccactaga actatagcta
     1141 gagtcctgg
//
