LOCUS       sequence-90081-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing 13 copies of the p53-binding 
            consensus sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, 
            Lin D, Mercer WE, Kinzler KW, Vogelstein B
  TITLE     WAF1, a potential mediator of p53 tumor suppression.
  JOURNAL   Cell. 1993 Nov 19. 75(4):817-25.
  PUBMED    8242752
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Nov 19. 75(4):817-25."
COMMENT     Sequence Label: PG13-luc (wt p53 binding sites)
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..119
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     103..119
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(609..625)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(610..626)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(650..669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(651..669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(676..693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(676..692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(685..707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(921..940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaggatagaa tggcgccggg cctttcttta tgtttttggc gtcttccatt ttaccaacag
       61 taccggaatg ccaagctcga gctccaccgc ggtggcggcc gctctagaac tagtggatcc
      121 cccgacggta tcgataagct tgtcgagggt ggtgaggctg aaatgaggcg ggaagaggcg
      181 gttggggctt aattatatca atttgggtgg ccccacagcg cctccaaggc gccagtcctg
      241 ttttgacaag ttgcctctgg aatgcctctc ttctttttct ccagagtaag cggaggccag
      301 gggcccccgg cctctgctta atactaaaaa aaacagatcg aattcgatcc tgcctggact
      361 tgcctggcct gcctggactt gcctggcctg cctggacttg cctggccctg cctggacttg
      421 cctggcctgc ctggacttgc ctggcctgcc tggacttgcc tggcctgcct ggacttgcct
      481 ggcctgcctg gacttgcctg gcctgcctgg acttgcctgg cctgcctgga cttgcctggc
      541 ctgcctggac ttgcctggcc tgcctggact tgcctggcct gcctggactt gcctggcatc
      601 aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
      661 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
      841 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      901 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      961 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1021 acggcacctc gaccccaaaa aacttgatta ggg
//
