LOCUS       sequence-90083-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..282
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        452..556
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        558..749
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(581..601)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 taaccctcac taaagggaac aaaagctgga gctgatttaa ataggagatc atgcatccag
       61 atctggtgat aggtggcaag tggtattccg taaggatatc ggatcttttc tcgactagta
      121 ctgacggaca caccgaagcc ccggcggcaa ccctcagcgg atgccccggg gcttcacgtt
      181 ttcccaggtc agaagcggtt ttcgggagta gtgccccaac tggggtaacc tttgagttct
      241 ctcagttggg ggcgtagggt cgccgacatg acacaagggg ttgtgaccgg ggtggacacg
      301 tacgcgggtg cttacgaccg tcagtcgcgc gagcgcgact agtcgagaag gatcctttgt
      361 cgacgaagtt cctattccga agttcctatt cttcaaaagg tataggaact tcgctagcga
      421 ttattcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      481 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      541 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
      601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      661 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      721 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      781 ccgcccagtt ccg
//
