LOCUS       sequence-90091-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(250..269)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    294..341
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
       61 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
      121 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
      181 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
      241 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
      301 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
      361 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
      421 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
      481 cgtcggggcg gcaggccctg ccatagcctc aggttactca ggatcatctc tagcctgcag
      541 gtgtgccacc atgcctggcc cagtttcttg ctttgtatta taaactttat agctggtaag
      601 actctggacc cacacgtgtt tgatttgtct cttctctcct gagacatttt ttcccagtaa
      661 catttcctga attttgtatt ttattgtctc tgcatctgtg ctgagacata acatcttctg
      721 tttcttctat ttgccatgaa ctgggagtta agtctaa
//
