LOCUS       sequence-90093-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter construct containing three hypoxia response 
            elements (24-mers) from the Pgk-1 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS
  TITLE     PTEN regulates p300-dependent hypoxia-inducible factor 1 
            transcriptional activity through Forkhead transcription factor 3a 
            (FOXO3a).
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7.
  PUBMED    18268343
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Feb 19. 105(7):2622-7."
COMMENT     Sequence Label: HRE-luciferase
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(114..176)
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     polyA_signal    377..498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(388..407)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     442..461
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(825..846)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctctagagga tagaatggcg ccgggccttt ctttatgttt ttggcgtctt ccattttacc
       61 aacagtaccg gaatgccaag cttacttaga tctgcggcac gctgttgacg ctgttaagcg
      121 ggtcgctgca gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg
      181 acctcggacc gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc
      241 tgggcggggc cggatctcga gctagcgtcg tgcaggacgt gacaactaga gtcgtgcagg
      301 acgtgacaac tagagtcgtg caggacgtga cagagctcgg tacctcccgg gttatgttag
      361 ctcagttaca gtaccataag atacattgat gagtttggac aaaccacaac tagaatgcag
      421 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      481 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      541 gaggtgtggg aggtttttta aagcaagtaa aacctccacg taccttaata ttacttactt
      601 atcatggtag cttgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      661 agttgcgcag cctgaatggc gaatggcaaa ttgtaagcgt taatattttg ttaaaattcg
      721 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      781 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      841 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      901 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      961 cactaaatcg gaacccta
//
