LOCUS       sequence-90095-e        7080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mouse Nox3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Banfi B, Malgrange B, Knisz J, Steger K, Dubois-Dauphin M, Krause KH
  TITLE     NOX3, a superoxide-generating NADPH oxidase of the inner ear.
  JOURNAL   J Biol Chem. 2004 Oct 29;279(44):46065-72. Epub 2004 Aug 23.
  PUBMED    15326186
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-29"; volume: "279(44)"; pages: "46065-72. 
            Epub 2004 Aug 23"
COMMENT     Sequence Label: pcDNA3.1-mNox3
FEATURES             Location/Qualifiers
     source          1..7080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2674..2691)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2680..2904
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2950..3378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3037..3056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3247..3268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3387..3407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3392..3721
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3572..3707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3634..3653
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3788..4582
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3842..3861)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4452..4471
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4756..4877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4793..4812)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4847..4866
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4926..4942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4926..4942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4939..4961)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4950..4966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4974..4980,4981..4998,4999..5004))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5019..5040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5157..5174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5328..5913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5408..5427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6084..6875,6876..6944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6707..6726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6945..7049)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgccggt gtgctggatt ctgaacgaga gtgggtcctt
      961 cgtggttgct ctcttatggc tggcagtaaa cgcctatctg tttattgaca cattcttctg
     1021 gtatactgaa gaggaggctt tcttttatac acgagttatt ctgggttccg cattggcatg
     1081 ggcccgggca tctgccgtgt gcctgaattt taactgcatg ctaattctgt tacctgtcag
     1141 tcggaacttc atttcactgg tgagaggaac aagtgtgtgc tgtagaggac catggagaag
     1201 acaactagac aaaaacctca acttccacaa actcgttgcc tacgggatag ctgtcaattc
     1261 agttatccac attgtggcac acttgttcaa cctggagcgt tatcacctgg gtcaggccaa
     1321 ggatgctgaa gggctgctgg ctgcactttc caaacttggc gatgccccaa atgagagcta
     1381 cctcaatcca gtccgcacct tttatatggg cacaaccact gagctattga tgacagtgtc
     1441 aggaattact ggcctgggta tctctctggc tctggtcttc atcatgacct cttcaaccga
     1501 attcatcaga aggtcctctt atgagctctt ctggtacaca caccatatct ttgtcttctt
     1561 cttcatcagt ctggccatcc acggaggagg tcgcatcatt cgaggccaaa ctccagagag
     1621 tctccggctg cacaatgtca cgtactgcag agaccactat gctgaatggc aggcagctgc
     1681 cttatgccct gtacctcaat tttctggcaa ggaaccttcg gcctggaaat gggctttggg
     1741 tcctgtggtc ttgtatgcgt gtgaaagaat aattaggttc tggagatctc accaagaagt
     1801 tgtcattacc aaggtggtga gtcacccatc tgcagtcctg gaacttcaca tgaagaagcg
     1861 agacttcaag atggcacctg gacagtacat cttcatccag tgcccatctg tctcccccct
     1921 ggagtggcac cccttcactc tcacctccgc tccccaggag gacttcttca gtgtacacat
     1981 cagagcctca ggagactgga cagaggcgtt attgaaggcc tttagagtag agggacaggc
     2041 tcccagtgag ctctgtagca tgccgaggct agcagtggat gggccctttg gaggctctct
     2101 ggcagatgta tttcactacc ccgtgagcgt gtgcattgca acgggaattg gagtcactcc
     2161 cttcgcctct cttctgaagt ctgtgtggta taagtgttgt gaatcacaga gcctgcctga
     2221 gctgagcaag gtgtacttct attggatctg ccgggatgcc ggagcatttg agtggtttgc
     2281 tgatctgtta ctgtcactgg aaacacggat gagtgaacaa gggaaggctc atttactgag
     2341 ctaccatata tatctcactg gctgggatga aaaccaggca attcacatag ctttacactg
     2401 ggatgaaagt ctggatgtga taacaggctt aaagcagaag gctttctatg ggcgacccaa
     2461 ctggaacgac gaattcaagc agattgccta caatcacccc agcagcagca ttggcgtgtt
     2521 cttctgtgga tccaaagcca tgtcaaagac tcttcaaaag atgtgtcgtt tgtactcatc
     2581 tgtggatccg aggggcgttc atttctatta caacaaggaa aacttctagg cggccgctcg
     2641 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     2701 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2761 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2821 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2881 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     2941 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3001 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3061 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3121 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3181 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3241 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3301 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3361 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3421 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3541 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3601 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3661 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3721 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     3781 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3841 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3901 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     3961 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4021 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4081 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4141 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4201 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4261 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4321 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4381 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4441 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4501 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4561 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4621 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4681 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4741 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4861 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     4921 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4981 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5041 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5761 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
     5881 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5941 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6001 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6061 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6121 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6181 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6241 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6301 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6361 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6421 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6481 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6541 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6601 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6661 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6721 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6781 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6841 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6901 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6961 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7021 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
