LOCUS       sequence-90102-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C T-Saphire
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(69..71,72..74,75..785)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(114..135)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(375..394)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     722..743
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(875..891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(875..891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(888..910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    899..915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(923..929,930..947,948..953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    968..989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcgcgaatg catctagata tcggatcccg ggcccgtcga ctgcagaggt acgggtctca
       61 tctgaacaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      241 ggcccaccct cgtgaccacc ttctcctacg gcgtgatggt gttcgcccgc taccccgacc
      301 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      481 tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcatg gccgacaagc
      541 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
      601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      661 acaaccacta cctgagcatc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      721 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      781 acaagggtgg aggaggttct ggaggcggtg gaagtggtgg cggaggtagc ggcacctgag
      841 acctctgcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttc
//
