LOCUS       sequence-90106-e        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac His6 MBP Asn10 TEV cloning vector wit...
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..97
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             217..234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtatactccg gactattaat agatcatgga gataattagc ggccgcgttt aaacatccac
       61 catttgatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
      121 ttcgtaacag ttttgtaata aaaaaaccta taactattcc ggattattca taccgtccca
      181 ccatcgggcg cggatcccgg accgatgggt tcttctcacc atcaccatca ccatggttct
      241 tctatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa aggctataac
      301 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
      361 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
      421 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
      481 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
      541 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
      601 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
      661 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
      721 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
      781 aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg
      841 attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat
      901 aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc
      961 aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc
     1021 gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag
     1081 ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggtta
//
