LOCUS       sequence-90112-e        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac StrepII msfGFP TEV cloning vector wit...
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(269..289)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(join(299..1009,1010..1012))
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(341..362)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     949..970
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1028..1051)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
       61 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      121 gattatgatc ctctagtact tctcgacaag cttggtaccg catgcctcga gtgcggcccc
      181 taggagatca tgaggcccct aggagatcat gaggccgcaa gcttgtcgac ggcgctcgaa
      241 ttcggatccg ttatccactt ccaatattgg attggaagta caggttttcc tcgatcccct
      301 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      361 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
      421 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      481 gctgcacgct gccgtcctcg acgttgtggc ggatcttgaa gttcgccttg atgccgttct
      541 tctgcttgtc ggccgtgata tagacgttgt ggctgttgaa gttgtactcc agcttgtgcc
      601 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      661 tgtcgccctc gaacttcacc tcggcgcggg tcttgtaggt gccgtcgtcc ttgaaggaga
      721 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      781 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
      841 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgttgg
      901 tggcatcgcc ctcgccctcg ccgcgcacgc tgaacttgtg gccgtttacg tcgccgtcca
      961 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc acagaagaac
     1021 cggcgccttt ttcgaactgc gggtggctcc agctagccat cggaccggga tccgcgcccg
     1081 atggtgggac ggtatgaata atccggaata gttataggtt tt
//
