LOCUS       sequence-90113-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac StrepII msfGFP TEV cloning vector wit...
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(190..195,196..212,213..218))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     627..644
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             761..784
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(842..863)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
       61 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      181 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
      241 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
      301 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
      361 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
      421 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
      481 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
      541 tggctacgta tactccggac tattaataga tcatggagat aattagcggc cgcgtttaaa
      601 catccaccat ttgatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
      661 tactgttttc gtaacagttt tgtaataaaa aaacctataa ctattccgga ttattcatac
      721 cgtcccacca tcgggcgcgg atcccggtcc gatggctagc tggagccacc cgcagttcga
      781 aaaaggcgcc ggttcttctg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      841 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga
      901 gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      961 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1021 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1081 ggagcgcacc atctccttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt
     1141 cgagggcga
//
