LOCUS       sequence-90115-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TwinStrepII Prescission SNAPf TEV clo...
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(290..310)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(875..898)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(899..922)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(959..982)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagtactt ctcgacaagc ttggtaccgc atgcctcgag actgcaggct
      181 ctagattcga aagcggcccc taggagatca tgaggccgcg actagtgagc tcgtcgacgt
      241 aggcctttga attccgcgcg cttcggaccg gttatccact tccaatattg gattggaagt
      301 acaggttttc accaccggcg ggtccaaggc caggtttgcc cagacgatgg ccctcatgtg
      361 ccaacagcca ttctttcaca gccagacctc cctcgtaacc tcccacgtcc aggtcgcctt
      421 ggacgactct atgacaaggg atcaggatag gtacagggtt gccactgagt gcggtcttaa
      481 cggcagcagt agcggctggg ttgcccgcca gggcggccaa gtgactgtat gagatcactt
      541 caccaaactt tacgaccttc aggagcttcc acaggacctg gcgggtgaag gattcctgtt
      601 ggaaaaccgg atgatggagc gcgggaacgg ggaactcctc gatcgcctct ggctgatgga
      661 aatatgcatt cagccaagcg gtagcttgca tcaaaggctc gggaccacca agcacggcag
      721 caggagccgg gacctcgact gcatctgctg cactagttcc ctttcccaga aagatgatac
      781 gatgcagacc ttgctcacaa ccgctcagtt ccaatttgcc aaggggcgaa tcgagcgtcg
      841 tgcgcttcat ctcgcagtct ttatcgccgc ttcccgggcc ctggaacagt acctccaatt
      901 tctcgaattg tgggtgtgac cagctgccac ctccgctacc gccgccgctg ccaccgcctt
      961 tctcaaattg ggggtgagac catgaagcca tggatccgcg cccgatggtg ggacggtatg
     1021 aataatccgg aatagttata ggtttt
//
