LOCUS       sequence-90124-e       10517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a 
            C-terminal GFP his tagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Parker JL, Newstead S
  TITLE     Molecular basis of nitrate uptake by the plant nitrate transporter 
            NRT1.1.
  JOURNAL   Nature. 2014 Mar 6;507(7490):68-72. doi: 10.1038/nature13116. Epub 
            2014 Feb 26.
  PUBMED    24572366
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13116"; journalName: "Nature"; date: "2014-03-6- 6"; 
            volume: "507"; issue: "7490"; pages: "68-72"
COMMENT     Sequence Label: pDDGFP2_leu2d_NRT1.1
FEATURES             Location/Qualifiers
     source          1..10517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3623..4064
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3623..3740
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4035..4058
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     4079..4095
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4112..4128
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5906..5926
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5936..5956
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5963..6673
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             6674..6697
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(6727..6743)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6728..6744)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6762..6780)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6982..7001)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6983..7001)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7011..7028)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7011..7027)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7020..7042)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7259..7278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(7679..8773)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     9009..9030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9298..10101)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10102..10322)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10348..10367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10467..10489
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cacggattag aagccgccga gcgggtgaca gccctccgaa
     3661 ggaagactct cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg
     3721 cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga
     3781 agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatgaacgaa tcaaattaac
     3841 aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg
     3901 aagcgatgat ttttgatcta ttaacagata tataaatgca aaaactgcat aaccacttta
     3961 actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc
     4021 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccggattc
     4081 tagaactagt ggatcccccc ccaccccgga ttctagaact agtggatccc ccatgtctct
     4141 tcctgaaact aaatctgatg atatccttct tgatgcttgg gacttccaag gccgtcccgc
     4201 cgatcgctca aaaaccggcg gctgggccag cgccgccatg attctttgta ttgaggccgt
     4261 ggagaggctg acgacgttag gtatcggagt taatctggtg acgtatttga cgggaactat
     4321 gcatttaggc aatgcaactg cggctaacac cgttaccaat ttcctcggaa cttctttcat
     4381 gctctgtctc ctcggtggct tcatcgccga tacctttctc ggcaggtacc taacgattgc
     4441 tatattcgcc gcaatccaag ccacgggtgt ttcaatctta actctatcaa caatcatacc
     4501 gggacttcga ccaccaagat gcaatccaac aacgtcgtct cactgcgaac aagcaagtgg
     4561 aatacaactg acggtcctat acttagcctt atacctcacc gctctaggaa cgggaggcgt
     4621 gaaggctagt gtctcgggtt tcgggtcgga ccaattcgat gagaccgaac caaaagaacg
     4681 atcgaaaatg acatatttct tcaaccgttt cttcttttgt atcaacgttg gctctctttt
     4741 agctgtgacg gtccttgtct acgtacaaga cgatgttgga cgcaaatggg gctatggaat
     4801 ttgcgcgttt gcgatcgtgc ttgcactcag cgttttcttg gccggaacaa accgctaccg
     4861 tttcaagaag ttgatcggta gcccgatgac gcaggttgct gcggttatcg tggcggcgtg
     4921 gaggaatagg aagctcgagc tgccggcaga tccgtcctat ctctacgatg tggatgatat
     4981 tattgcggcg gaaggttcga tgaagggtaa acaaaagctg ccacacactg aacaattccg
     5041 ttcattagat aaggcagcaa taagggatca ggaagcggga gttacctcga atgtattcaa
     5101 caagtggaca ctctcaacac taacagatgt tgaggaagtg aaacaaatcg tgcgaatgtt
     5161 accaatttgg gcaacatgca tcctcttctg gaccgtccac gctcaattaa cgacattatc
     5221 agtcgcacaa tccgagacat tggaccgttc catcgggagc ttcgagatcc ctccagcatc
     5281 gatggcagtc ttctacgtcg gtggcctcct cctaaccacc gccgtctatg accgcgtcgc
     5341 cattcgtcta tgcaaaaagc tattcaacta cccccatggt ctaagaccgc ttcaacggat
     5401 cggtttgggg cttttcttcg gatcaatggc tatggctgtg gctgctttgg tcgagctcaa
     5461 acgtcttaga actgcacacg ctcatggtcc aacagtcaaa acgcttcctc tagggtttta
     5521 tctactcatc ccacaatatc ttattgtcgg tatcggcgaa gcgttaatct acacaggaca
     5581 gttagatttc ttcttgagag agtgccctaa aggtatgaaa gggatgagca cgggtctatt
     5641 gttgagcaca ttggcattag gctttttctt cagctcggtt ctcgtgacaa tcgtcgagaa
     5701 attcaccggg aaagctcatc catggattgc cgatgatctc aacaagggcc gtctttacaa
     5761 tttctactgg cttgtggccg tacttgttgc cttgaacttc ctcattttcc tagttttctc
     5821 caagtggtac gtttacaagg aaaaaagact agctgaggtg gggattgagt tggatgatga
     5881 gccgagtatt ccaatgggtc atggggaaaa tttatatttt caaggtcaat ttggggaaaa
     5941 tttatatttt caaggtcaat tttctaaagg tgaagaatta ttcactggcg ttgtcccaat
     6001 tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga
     6061 aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc
     6121 agttccatgg ccaaccttag tcactacttt cggttatggt gttcaatgtt ttgcgagata
     6181 cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca
     6241 agaaagaact atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt
     6301 tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg
     6361 taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc
     6421 tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     6481 ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt
     6541 gttaccagac aaccattact tatccactca atctgcctta tccaaagatc caaacgaaaa
     6601 gagagaccac atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga
     6661 tgaattgtac aaacaccacc accatcacca tcatcattaa ctgcaggaat tcgatatcaa
     6721 gcttatcgat accgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc
     6781 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     6841 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     6901 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     6961 aaggttttgg gacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7021 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7081 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7141 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg
     7201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gcaaatggtc aggtcattga
     7321 gtgtttttta tttgttgtat tttttttttt ttagagaaaa tcctccaata tcaaattagg
     7381 aatcgtagtt tcatgatttt ctgttacacc taactttttg tgtggtgccc tcctccttgt
     7441 caatattaat gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt
     7501 tgcttacctg tattccttta ctatcctcct ttttctcctt cttgataaat gtatgtagat
     7561 tgcgtatata gtttcgtcta ccctatgaac atattccatt ttgtaatttc gtgtcgtttc
     7621 tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga gaatcttttt
     7681 aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg gtactgttgg
     7741 aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa
     7801 tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca gcagacaaga
     7861 tagtggcgat agggtcaacc ttattctttg gcaaatctgg agcagaaccg tggcatggtt
     7921 cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca gatggcaaca
     7981 aacccaagga acctgggata acggaggctt catcggagat gatatcacca aacatgttgc
     8041 tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat
     8101 caatcaattg atgttgaacc ttcaatgtag ggaattcgtt cttgatggtt tcctccacag
     8161 tttttctcca taatcttgaa gaggccaaaa gattagcttt atccaaggac caaataggca
     8221 atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt tgcacttctg
     8281 gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc tttctcttac
     8341 caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt
     8401 gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat ggtcttaagt
     8461 tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt ctaacactac
     8521 cggtacccca tttaggacca gccacagcac ctaacaaaac ggcatcaacc ttcttggagg
     8581 cttccagcgc ctcatctgga agtgggacac ctgtagcatc gatagcagca ccaccaatta
     8641 aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct ttaagaacct
     8701 taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct
     8761 taggggcaga cattacaatg gtatatcctt gaaatatata taaaaaaagg cgccttagac
     8821 cgctcggcca aacaacgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8881 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8941 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9001 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9061 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9121 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat
     9181 tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta
     9241 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
     9301 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
     9361 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
     9421 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
     9481 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
     9541 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
     9601 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
     9661 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
     9721 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
     9781 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     9841 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     9901 atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     9961 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
    10021 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
    10081 cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag
    10141 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
    10201 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
    10261 aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg
    10321 aaaagctgtg gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10381 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
    10441 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
    10501 atcaccgaaa cgcgcga
//
