LOCUS       sequence-90125-e        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for expressing recombinant proteins as a C terminal GFP-His 
            tagged constuct in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Parker JL, Newstead S
  TITLE     Method to increase the yield of eukaryotic membrane protein 
            expression in Saccharomyces cerevisiae for structural and functional
            studies.
  JOURNAL   Protein Sci. 2014 Jun 20. doi: 10.1002/pro.2507.
  PUBMED    24947543
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2014 Jun 20. doi: 10.1002/pro.2507."
COMMENT     Sequence Label: pDDGFP_LEU2D
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3623..4064
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3623..3740
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4035..4058
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     4079..4095
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4079..4095
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4106..4126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4133..4843
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4844..4867
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(4897..4913)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4898..4914)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4932..4950)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5154..5173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5155..5173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5183..5200)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5183..5199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5192..5214)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5431..5450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5851..6945)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     7181..7202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7470..8273)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8274..8494)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8520..8539)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8639..8661
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cacggattag aagccgccga gcgggtgaca gccctccgaa
     3661 ggaagactct cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg
     3721 cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga
     3781 agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatgaacgaa tcaaattaac
     3841 aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg
     3901 aagcgatgat ttttgatcta ttaacagata tataaatgca aaaactgcat aaccacttta
     3961 actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc
     4021 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccggattc
     4081 tagaactagt ggatcccccc ccggggaaaa tttatatttt caaggtcaat tttctaaagg
     4141 tgaagaatta ttcactggcg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg
     4201 tcacaaattt tctgtctccg gtgaaggtga aggtgacgct acttacggta aattgacctt
     4261 aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt
     4321 cggttatggt gttcaatgtt ttgcgagata cccagatcat atgaaacaac atgacttttt
     4381 caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg
     4441 taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga
     4501 attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa
     4561 ctataactct cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaagttaa
     4621 cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa ttagctgacc attatcaaca
     4681 aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact tatccactca
     4741 atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt tagaatttgt
     4801 tactgctgct ggtattaccc atggtatgga tgaattgtac aaacaccacc accatcatca
     4861 tcatcattaa ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagtcatg
     4921 taattagtta tgtcacgctt acattcacgc ccctcccccc acatccgctc taaccgaaaa
     4981 gggaaggagt tagacaacct gaagtctagg tcccctattt atttttttat agttatgtta
     5041 gtattaagaa cgttatttat attcaaattt tccttttttt tctgtacaga cgcgtgtacg
     5101 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacccaat tcgccctata
     5161 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5221 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     5341 gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5461 ccacgttcgc cggcaaatgg tcaggtcatt gagtgttttt tatttgttgt attttttttt
     5521 ttttagagaa aatcctccaa tatcaaatta ggaatcgtag tttcatgatt ttctgttaca
     5581 cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt gcaattcttt
     5641 ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt tactatcctc
     5701 ctttttctcc ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga
     5761 acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat
     5821 gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc
     5881 gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc
     5941 tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa
     6001 tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggtcaa ccttattctt
     6061 tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc
     6121 tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc
     6181 ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt
     6241 tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt
     6301 agggaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa
     6361 aagattagct ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa
     6421 agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc
     6481 gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct
     6541 gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag
     6601 agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat
     6661 ttttagtaaa ccttgttcag gtctaacact accggtaccc catttaggac cagccacagc
     6721 acctaacaaa acggcatcaa ccttcttgga ggcttccagc gcctcatctg gaagtgggac
     6781 acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt
     6841 ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc
     6901 aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacattacaa tggtatatcc
     6961 ttgaaatata tataaaaaaa ggcgccttag accgctcggc caaacaacgc cggctttccc
     7021 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7081 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7141 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7201 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7261 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7321 atattaacgt ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7381 cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
     7441 atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat
     7501 gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca
     7561 aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt
     7621 ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc
     7681 aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca
     7741 aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg
     7801 gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct
     7861 tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta
     7921 atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta
     7981 ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc
     8041 tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt
     8101 agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga
     8161 acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca
     8221 gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat
     8281 cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat
     8341 gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     8401 tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa
     8461 aaagaataaa aaaaaaatga tgaattgaat tgaaaagctg tggtatggtg cactctcagt
     8521 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8581 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8641 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
