LOCUS       sequence-90128-e        1449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the POT family transporter from Streptococcus thermophilus
            in E. coli as a C-terminally tagged GFP his tagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S
  TITLE     Alternating access mechanism in the POT family of oligopeptide 
            transporters.
  JOURNAL   EMBO J. 2012 Aug 15;31(16):3411-21. doi: 10.1038/emboj.2012.157. 
            Epub 2012 Jun 1.
  PUBMED    22659829
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.157"; journalName: "EMBO J"; date: "2012-08-15- 
            15"; volume: "31"; issue: "16"; pages: "3411-21"
COMMENT     Sequence Label: pWaldo-GFPd_PepTSt
FEATURES             Location/Qualifiers
     source          1..1449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaagaca aaggaaagac atttttcggg caacctttag gactgtcaac actattcatg
       61 actgaaatgt gggagcgttt ctcatactat ggtatgcgcg ccatcttgct ttactatatg
      121 tggttcctca tcagtactgg tgatctacac attacacgag caacagccgc atcaatcatg
      181 gcaatttacg cttcaatggt ttacctttct ggtacgatcg gtggtttcgt agccgatcgt
      241 atcatcggag cacgtccagc tgtattctgg ggtggtgttc tcatcatgct tggacatatc
      301 gtgctcgctt tgccttttgg cgcaagcgca ttgtttgggt caatcatctt aattattata
      361 ggaactggtt tcttgaagcc aaacgtttca acattggttg ggacattgta tgacgaacat
      421 gatcgtcgtc gtgacgctgg tttctcaatc tttgtgtttg gtattaactt gggtgcgttc
      481 attgcaccac ttattgttgg tgctgcacaa gaagctgcag gttaccacgt ggccttctca
      541 ttggcagcaa tcggtatgtt tattggtttg ctggtttatt acttcggtgg taagaaaaca
      601 ttggatccgc attacttgcg cccaactgat ccactagcac cagaagaagt taagccatta
      661 ctggttaagg taagcttagc tgttgctggc tttattgcaa tcatcgttgt gatgaacctt
      721 gtcggttgga attcattacc tgcctacatc aatctcttga cgattgttgc gatcgccatt
      781 ccggttttct attttgcttg gatgatttct tcagtgaagg tgacgtcaac tgaacatttg
      841 cgtgttgtgt cttacatccc acttttcatc gctgctgttt tgttctgggc gattgaagaa
      901 caaggctcag ttgtgttggc aacatttgct gctgaacgtg ttgattcatc ttggttccct
      961 gtctcatggt tccaatcatt gaacccactg ttcatcatgc tttacacacc attcttcgct
     1021 tggctatgga ctgcctggaa gaaaaaccaa ccatcatcac caacaaagtt tgccgttggt
     1081 ttgatgtttg ccggcttaag cttcttgcta atggccattc ctggtgcttt gtatggcaca
     1141 agtggtaaag tatcaccact atggttagtt ggttcatggg cccttgttat cctcggtgaa
     1201 atgttaattt cacctgtcgg cctatccgtc acaactaagt tggcaccaaa agcctttaat
     1261 tctcaaatga tgtcaatgtg gttcttgtca tcatcggtag gttctgcttt gaacgcacaa
     1321 ctggtgacgt tgtataacgc caaatctgaa gtcgcttact tctcttactt cggattaggt
     1381 tctgttgtac ttggtattgt tctcgtcttc ctatcaaagc gtattcaagg tttgatgcaa
     1441 ggtgttgaa
//
