LOCUS       sequence-90133-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C 6xHIS
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..83)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(150..167)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(150..166)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..181)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(375..394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     494..516
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(554..572)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccggtctc tggtgctgga tccgccgctg
       61 ccgccgtgat ggtgatggtg atgcattgtt cagatgagac catgggatcc gatatctaga
      121 tgcattcgcg aggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
      181 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      241 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      301 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      361 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
      421 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      481 gctgtgacct tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      541 gcgagacaaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gagcggcgtt
      601 gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt
      661 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
      721 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
      781 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
      841 ggtgctc
//
