LOCUS       sequence-90138-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C HA
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..92)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(159..176)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(159..175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(384..403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     503..525
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(563..581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccggtctc tggtgctgga tccgccgctg
       61 ccgccagcgt aatctggaac atcgtatggg tacattgttc agatgagacc atgggatccg
      121 atatctagat gcattcgcga ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
      181 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      241 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      301 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      361 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      421 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      481 tacagacaag ctgtgacctt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      541 ccgaaacgcg cgagacaaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      601 agcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa
      661 cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc
      721 ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca
      781 cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc
      841 cctgcctccg g
//
