LOCUS       sequence-90140-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C N-NES KOZ b c7
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(191..208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(191..207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(200..222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(416..435)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     535..557
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(595..613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccgggatc cgatatctag atgcattcga
       61 tggtctcatc tgaacaatgc tgcagaacga gcttgctctt aagttggctg gacttgatat
      121 taacaagact ggaggagtcg actcgagtgc ggccgccacc tgagacctac gaggtaccga
      181 gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      361 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      421 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc ttctccggga
      541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacaa aagggcctcg
      601 tgatacgcct atttttatag gttaatgtca tgagcggcgt tgtgacaatt taccgaacaa
      661 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
      721 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
      781 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
      841 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctc
//
