LOCUS       sequence-90146-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E c-myc
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..78)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(155..172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(155..171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(164..186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(380..399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     499..521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(559..577)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccggtctc tgattttaaa gatcctcctc
       61 agaaatcaac ttttgctcgg atccgccgct gccgcccttt gagaccatgg gatccgatat
      121 ctagatgcat tcgcgaggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
      181 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      241 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      301 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      361 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      421 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      481 gacaagctgt gaccttctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      541 aacgcgcgag acaaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgagcg
      601 gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa
      661 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
      721 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
      781 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
      841 cctccggtgc tc
//
