LOCUS       sequence-90154-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 600, Emission: 650.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mNeptune2-N1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..839
                     /codon_start=1
                     /product="far-red fluorescent protein with spectra similar 
                     to those of mNeptune but improved brightness"
                     /label=mNeptune2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTG
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg tctaagggcg
      121 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
      181 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccggca
      241 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctacctgct
      301 tcatgtacgg cagcaagacc ttcatcaacc acacccaggg catccccgat ttctttaagc
      361 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
      421 ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac gtcaagctca
      481 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      541 ccagtaccga gaccctgtac cccgctgacg gcggcctgga aggcagatgc gacatggccc
      601 tgaagctcgt gggcgggggc cacctgcact gcaacctgaa gaccacatac agatccaaga
      661 aacccgctaa gaacctcaag atgcccggcg tctactttgt ggaccgcaga ctggaaagaa
      721 tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
      781 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtaag
      841 cggccgcgac tctagatcat a
//
