LOCUS       sequence-90171-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta R 5-6
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(178..183,184..201,202..208)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(328..347)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggattacgga agacaatgtc gacatgagac caaagctgaa cgagaaacgt aaaatgatat
       61 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      121 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
      181 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      241 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      301 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      361 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      421 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      481 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      541 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      601 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      661 ttccctaaag ggtttattga gaatatgttt ttcgtgtcag ccaatccctg ggtga
//
