LOCUS       sequence-90173-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta R 5-6
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..370)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(167..186)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agaagacaac tcagcggtga gaccgtcgac ctgcagactg gctgtgtata agggagcctg
       61 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
      121 tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata tcggtggtca
      181 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat
      241 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
      301 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
      361 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
      421 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
      481 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
      541 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
      601 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
      661 gcgcaaatac gcatactg
//
