LOCUS       sequence-90179-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha F A-B
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(158..163,164..181,182..188)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(308..327)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgttgtctt caaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
       61 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      121 ggcggcccct cgaggggccg cattaggcac cccaggcttt acactttatg cttccggctc
      181 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      241 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      301 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      361 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      421 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
      481 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      541 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      601 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
      661 gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
      721 ggcc
//
