LOCUS       sequence-90181-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha R A-B
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..404)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(201..220)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atatatcctg tcaaacactg atagtttaaa ccagaggtct cacagatacg aagtcttcgt
       61 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
      121 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      181 acggaaaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      241 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg
      301 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
      361 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
      421 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
      481 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
      541 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
      601 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
      661 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
      721 cttttagtaa gccggatcct ctagattacg ccccgccctg ccactcatcg
//
