LOCUS       sequence-90182-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha R E-F
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(140..145,146..163,164..170)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(290..309)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gaacgaaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
       61 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
      121 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
      181 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      241 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      301 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
      361 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
      421 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
      481 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
      541 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
      601 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc
      661 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggac
//
