LOCUS       sequence-90186-e         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha R 3-4
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(131..436)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLXSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINXMFWGI"
     primer_bind     complement(233..252)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ataacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aaccagagaa gacaagacag cggtgagacc gtcgacctgc agactggctg tgtataaggg
      121 agcctgacat ttatattccc cagaacatca ngttaatggc gtttttgatg tcattttcgc
      181 ggtggctgag atcagccact tcttccccga taacggaaac cggcacactg gccatatcgg
      241 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacggggga
      301 ctttatctga cancagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
      361 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
      421 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcan caaaagagcc gttcatttca
      481 ataaaccggg cgacctcagc catcccttcc tgattttcc
//
