LOCUS       sequence-90187-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha F 4-5
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(186..191,192..209,210..216)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(336..355)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 actggggtgg attacggaag acaatgtcgc ggtgagacca aagctgaacg agaaacgtaa
       61 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
      121 tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca ttaggcaccc
      181 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
      241 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      301 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      361 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      421 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      481 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      541 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      601 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      661 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtgtcagcc aatccctggg
      721 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
      781 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
      841 ttcatcatgc cgtttgtgat gg
//
