LOCUS       sequence-90188-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha R 5-6
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(197..202,203..220,221..227)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(347..366)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtttttaatg tactggggtg gattacggaa gacaatgagg acatgagacc aaagctgaac
       61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      121 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggcccctc gaggggccgc
      181 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      241 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      301 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      361 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      421 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      481 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      541 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      601 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      661 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtgtcagc
      721 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
      781 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
      841 ggcgattcag gttcatcatg
//
