LOCUS       sequence-90189-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha F 1-2
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(199..204,205..222,223..229)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(349..368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgtttttaa tgtactgggg tggattacgg aagacaatac ggcggtgaga ccaaagctga
       61 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      121 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      181 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      241 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      301 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      361 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      421 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      481 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      541 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      601 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      661 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtgtca
      721 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      781 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      841 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      901 gaattacaac agtactgc
//
