LOCUS       sequence-90190-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta R 1-2
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..40
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(126..431)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(228..247)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaacca
       61 gagaagacaa gcccgcggtg agaccgtcga cctgcagact ggctgtgtat aagggagcct
      121 gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg
      181 ctgagatcag ccacttcttc cccgataacg gaaaccggca cactggccat atcggtggtc
      241 atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg ggggacttta
      301 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata
      361 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
      421 ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca tttcaataaa
      481 ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg
      541 acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc
      601 ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcatac
      661 ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag
      721 cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatcctcta gattacgccc
      781 cgccctgcca ctcatcgcag tactg
//
