LOCUS       sequence-90194-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter assay for RECK 3'UTR that has point mutations on
            miR-21 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Jung HM, Phillips BL, Patel RS, Cohen DM, Jakymiw A, Kong WW, Cheng 
            JQ, Chan EK
  TITLE     Keratinization-associated miR-7 and miR-21 regulate tumor suppressor
            reversion-inducing cysteine-rich protein with kazal motifs (RECK) in
            oral cancer.
  JOURNAL   J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 
            10.1074/jbc.M112.366518. Epub 2012 Jul 2.
  PUBMED    22761427
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.366518"; journalName: "J Biol Chem"; date: 
            "2012-08-24- 24"; volume: "287"; issue: "35"; pages: "29261-72"
COMMENT     Sequence Label: pMiR-RECK-3'UTR-miR-21-mut
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..809
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             821..850
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             869..892
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(912..928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(912..928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac tcactatagg
       61 gcggccggga atcgtcgact ggatccagta ccgaggagat ctgcgccgcg ttcgccatga
      121 gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
      181 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
      241 agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
      301 tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc
      361 agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc
      421 tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca
      481 acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg
      541 ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc
      601 tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga
      661 aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa
      721 tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact
      781 gcgacctccc tagcaaactg gggcacagat cgcgtccctc gagcagaaac tcatctcaga
      841 agaggatctg gcagcaaatg atatcctgga ttacaaggat gacgacgata aggtttaaac
      901 ggccggccgc ggtcatagct gtttcctgaa cagatcccgg gtggcatccc tgtgacccct
      961 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     1021 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
//
