LOCUS       sequence-9021-ex        3759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pDR3.13
FEATURES             Location/Qualifiers
     source          1..3759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1935..2034)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2146..2165)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2157..2966
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2224..2243)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3109..3697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3598..3617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatggcgac gaaagagcag attgttgagc accgcgagtc tgaaattcct
      481 atagccagcc agtggaaggc cacgtttccc gttgatctag agattgaaaa aaattctgaa
      541 atgtttgcgc ttcgctacat aaaatgtgct tcggccttca ttctcgatcg ccgtggcatt
      601 ttggacgaaa aatgcttcaa aactcgaaca ataggtatgg cttattttac aatgtatcac
      661 aaatattttt atacagataa actgttggtc accgcattcc agagcagtgt tcctgccgca
      721 aagcgtgtgt cttcaacatt cgacggattg agagatgcga ttcaacaagg ctatttgcga
      781 gagttcgcaa tcgtgttcta caagaagcca aacgaggaag acattaatga agtgtttgcg
      841 ttcaggtttg cttatggaga tgaaggagag attttcgtat ctctaaataa gtaagctgct
      901 ggtcttattc aattattcgt tttcaattgt tttcagcgga atcgacacga acgaaagcag
      961 tcaagaactg ttacaggcga aattcgttga taccgacaac acgaaacaaa tgtttgcaag
     1021 cacaatcaag aaacttcacc ggtgtatcaa gaaaatggag ccgcttcctc aagggtccga
     1081 cgcgagtttc cgtgtttcgt acactgaaag taggttacat tcacagcttt agtctatttc
     1141 taactgttat tttaagaagc tccaaaagac tatacacccg aaggctatct gctgagtcct
     1201 atgttctata cgctgaatca ggatattagg aaagcatcta ttggaatcgt gtgtggcggt
     1261 catcacaaaa ttcaaatgct tgctgcttca caatacttga agcaagactt cgatctggat
     1321 aaaacgacca cactcaatcc aaatatgtca atcatggcaa accaatcgaa aaggaaggga
     1381 cgaatttcca gagattcccc ttacggtctc agtcagggca ttacgaagaa gaataaggac
     1441 tagtcgaaaa gttttcactc atgtgcaatg tttctcttgt tttatatttt tagccattgt
     1501 ttttataatt tcattgattg tcgtgctttt gtcttctctt gtgtgatatc aatctatctt
     1561 cttcacgtat agtttcatct gtttgattga gttatttttc tttcactgta agtctggtgt
     1621 ttcgtagatt tagaaaaaag tagcgtaata aattattttg aaatggacca aacattcaaa
     1681 tccagttcag ataaatttat ttcattgaag cagtgttgga attcggacaa atgagaagtg
     1741 aatattcatt tgaaataagt tttacattac ttgcactgtt ggctgggtgt taaaaatagg
     1801 ttcaatattg aattaataaa atgggacaat tcaccgtgca gaaaaaaacg aaggaaaagg
     1861 cttaaaattg aagaagaagt ataaaagaat cacatgggct tttcaggaat tcagtaaatg
     1921 tttggacaag tataacccag ctttcttgta caaagtgggc attataagaa agcattgctt
     1981 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2041 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2101 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     2161 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     2221 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     2281 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     2341 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     2401 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     2461 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     2521 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     2581 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2641 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2701 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2761 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     2821 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     2881 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     2941 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     3001 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     3061 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3241 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3721 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
