LOCUS       sequence-9022-ex        3641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pDR3.10
FEATURES             Location/Qualifiers
     source          1..3641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1817..1916)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2028..2047)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2039..2848
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2106..2125)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2991..3579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3480..3499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatggccca atccgtccca ccaggagaca tccagaccca gccgggtaca
      481 aagattgtct tcaatgctcc gtacgacgac aagcacacgt atcacatcaa agtgatcaat
      541 tcgtcggcac gccgtattgg atacggtatc aagaccacca acatgaagag acttggagtt
      601 gatccaccat gtggagttct cgacccaaag gaagcagtgc ttctggcagt ttcctgcgat
      661 gcatttgctt ttggacaaga agacaccaac aacgaccgta tcaccgtcga atggaccaac
      721 accccggatg gagctgccaa gcagttccgc cgcgagtggt tccagggaga tggtatggtc
      781 cgtcgcaaga atctgccgat cgaatataac ccatgagaac ttctaaataa atattgtttt
      841 acctcattgt ttgttaattt ttaggattaa aatacattat ctgactagta gcccattctc
      901 ccaatatgga gttgaaaggt gccgccaaat ttggcggttt ctgagacctt gctatttctc
      961 agtgaagaat taataatgga gttagaacct tctcgaataa cgttccaagt ctttatattt
     1021 gataaaaaaa attttcaggt ttaagtttga gtttgagaat gatggagata gctttccaga
     1081 agtcacagat tccctgactt tgaatgtcat ttataggatt tcagttgagc tcggtgtcac
     1141 catgatcata cagaaacaag agttgcaaaa cttcggtgtg aactgatttt caaagaaaac
     1201 tgacgtttca gttgtttgca gtttgtggac taatgacgat cttctgaaca tgtgaaaaat
     1261 acgagtggtt tggaatattt gtaatcgaat agtaccccgc atactgacat ctttctatca
     1321 atttgttcca aaaatatgct agagaaacct gtgacaattt cccaagtgca gtattgaatt
     1381 ggctatggtt caatggaatt aggaagaaca agggattcat acgcttgctt tcattcatac
     1441 gctcgcatac gcttgaaact tacagcgttt ttgaatttac aagatccgga acttgaaagg
     1501 gtaccgattt ccatattttc ttaattagta tgtaatggaa taacctctat gctcacctaa
     1561 acatcaattt gaacactcca tggagagtga ggaatcgacc aaaatttttt cgagcaactt
     1621 caagtcattt gctatagagc tatgatagac cccaagcttt cttgcaatct tacggatact
     1681 ccgccacaac tttctgacac attctttctt ctgtttggaa acagtttaga atgtgcgaca
     1741 gttatatgta ggctgattgt gagagactca ttcacttaat tattaaatat acaaacaaat
     1801 atcatacaaa aatcgaaccc agctttcttg tacaaagtgg gcattataag aaagcattgc
     1861 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1921 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1981 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2041 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     2101 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     2161 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2221 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2281 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     2341 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2401 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2461 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2521 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2581 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2641 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2701 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2761 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2821 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     2881 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     2941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3121 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3601 ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
