LOCUS       sequence-90230-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human codon optimized Csy4 and FokI-dCas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pSQT1601
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgcgcagctg atgtctgtcc tttttggcaa acttcatcag gccctggttg cccagggcgg
       61 agatcggata ggggtaagct ttccagacct cgacgaaagc cggagccgcc tgggagaacg
      121 cctgcggatc cacgcttctg ccgacgatct gagagccttg ctggcaaggc catggcttga
      181 ggggctccgg gatcacctgc agtttggcga acccgccgtt gttccccacc caacccctta
      241 tcggcaggtg tctagagtgc aggccaaatc taatccagaa cggctgcgac ggcgactcat
      301 gcggcgacat gatcttagcg aggaagaggc ccgaaaaaga atccctgata ccgtggcccg
      361 cgcccttgac ttgccttttg tcacactgcg gtcccagagt acggggcagc atttcagact
      421 tttcattcga cacgggccac tgcaagttac cgccgaagaa ggaggcttta cttgttatgg
      481 actctccaag ggaggtttc
//
